DISCO-MS
An exceedingly straightforward and refined multi-omics endeavor!
Introduction:
"Here, we present DISCO-MS, a technology that combines whole-organ/whole-organism clearing and imaging, deep-learning-based image analysis, robotic tissue extraction, and ultra-high-sensitivity mass spectrometry."
Paper: https://lnkd.in/gAG4QX4D
Highlights:
• DISCO-MS is an AI and robotics assisted spatial proteomics technique for optically cleared whole specimens, yielding proteome akin to fresh samples.
• DISCO-bot-aided DISCO-MS identifies spatial immune cell heterogeneity in mouse bones and plaque heterogeneity in human coronary arteries.
Authors (shortened):
A combined effort by Harsharan S. Bhatia, PhD., Andreas-David Brunner, Furkan Ozturk, Dr Saketh Kapoor, PhD et al., in Cell Press
Affiliations:
Helmholtz Munich, Max Planck Institute of Biochemistry, Bruker, Ludwig-Maximilians-Universität München
Interests:
"F.T. reports receiving consulting fees from Roche Diagnostics GmbH and Cellarity, Inc., and ownership interest in Cellarity, Inc., and Dermagnostix. A.E., H.S.B., F.O., S.K., M.I.T., and M.N. have filed a patent related to robotics technologies presented in this work."
Video S5. DISCO-bot real-time extraction of ROIs from scapula of mouse, related Figure 5. Robotic arm guided real time extraction of ROIs from bone marrow of scapula in mouse. The procedure involves 4 major steps: 1) Finding the target of interest and approaching with needle 2) cutting through bone to reach the marrow 3) excising around the target and extracting the tissue with vacuum, and 4) examining the extracted area to ensure successful extraction.
